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Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2 in Preclinical Animal Models
Jun-Guy Park; Fatai S. Oladunni; Mohammed A. Rohaim; Jayde Whittingham-Dowd; James Tollitt; Bakri M Assas; Wafaa Alhazmi; Abdullah Almilaibary; Munir Iqbal; Pengxiang Chang; Renee Escalona; Vinay Shivanna; Jordi B. Torrelles; John J Worthington; Lucy H. Jackson-Jones; Luis Martinez-Sobrido; Muhammad Munir.
Afiliación
  • Jun-Guy Park; Texas Biomedical Research Institute, Host-Pathogen Interactions and Population Health Programs, San Antonio, TX, 78227, USA
  • Fatai S. Oladunni; Texas Biomedical Research Institute, Host-Pathogen Interactions and Population Health Programs, San Antonio, TX, 78227, USA
  • Mohammed A. Rohaim; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK
  • Jayde Whittingham-Dowd; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK
  • James Tollitt; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK
  • Bakri M Assas; Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, Immunology Group, King Abdul Aziz University, Jeddah, Saudi Arabia
  • Wafaa Alhazmi; Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, Microbiology Group, King Abdul Aziz University, Jeddah, Saudi Arabia
  • Abdullah Almilaibary; Faculty of Medicine, Al Baha University, Al Baha, Saudi Arabia
  • Munir Iqbal; The Pirbright Institute, UK
  • Pengxiang Chang; The Pirbright Institute, UK
  • Renee Escalona; Texas Biomedical Research Institute, Host-Pathogen Interactions and Population Health Programs, San Antonio, TX, 78227, USA
  • Vinay Shivanna; Texas Biomedical Research Institute, Host-Pathogen Interactions and Population Health Programs, San Antonio, TX, 78227, USA
  • Jordi B. Torrelles; Texas Biomedical Research Institute, Host-Pathogen Interactions and Population Health Programs, San Antonio, TX, 78227, USA
  • John J Worthington; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK
  • Lucy H. Jackson-Jones; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK
  • Luis Martinez-Sobrido; Texas Biomedical Research Institute, Host-Pathogen Interactions and Population Health Programs, San Antonio, TX, 78227, USA
  • Muhammad Munir; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-425974
ABSTRACT
The global deployment of an effective and safe vaccine is currently a public health priority to curtail the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based intranasal vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy in challenge studies with SARS-CoV-2. The recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 administrated via intranasal route in mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters vaccinated with two doses of vaccine showed complete protection from clinical disease including lung infection, inflammation, and pathological lesions after SARS-CoV-2 challenge. Importantly, a single or double dose of intranasal rNDV-S vaccine completely blocked SARS-CoV-2 shedding in nasal turbinate and lungs within 4 days of vaccine administration in hamsters. Taken together, intranasal administration of rNDV-S has the potential to control infection at the site of inoculation, which should prevent both the clinical disease and transmission to halt the spread of the COVID-19 pandemic.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Experimental_studies / Prognostic_studies Idioma: En Año: 2021 Tipo del documento: Preprint
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Experimental_studies / Prognostic_studies Idioma: En Año: 2021 Tipo del documento: Preprint