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Human B cell clonal expansion and convergent antibody responses to SARS-CoV-2
Sandra Cathrine Abel Nielsen; Fan Yang; Katherine JL Jackson; Ramona A. Hoh; Katharina Röltgen; Bryan Stevens; Ji-Yeun Lee; Arjun Rustagi; Angela J. Rogers; Abigail E. Powell; Javaria Najeeb; Ana Rita Otrelo-Cardoso; Kathryn E Yost; Bence Daniel; Howard Y Chang; Ansuman T Satpathy; Theodore S. Jardetzky; Peter S. Kim; Taia T. Wang; Benjamin A. Pinsky; Catherine A Blish; Scott D Boyd.
Afiliación
  • Sandra Cathrine Abel Nielsen; Stanford University
  • Fan Yang; Stanford University
  • Katherine JL Jackson; Garvan Institute of Medical Research
  • Ramona A. Hoh; Stanford University
  • Katharina Röltgen; Stanford University
  • Bryan Stevens; Stanford University School of Medicine
  • Ji-Yeun Lee; Stanford University
  • Arjun Rustagi; Stanford University
  • Angela J. Rogers; Stanford University
  • Abigail E. Powell; Stanford University
  • Javaria Najeeb; Stanford University
  • Ana Rita Otrelo-Cardoso; Stanford University
  • Kathryn E Yost; Stanford University
  • Bence Daniel; Stanford University
  • Howard Y Chang; Stanford University
  • Ansuman T Satpathy; Stanford University
  • Theodore S. Jardetzky; Stanford University
  • Peter S. Kim; Stanford University
  • Taia T. Wang; Stanford University School of Medicine
  • Benjamin A. Pinsky; Stanford University School of Medicine
  • Catherine A Blish; Stanford University
  • Scott D Boyd; Stanford University
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-194456
ABSTRACT
During virus infection B cells are critical for the production of antibodies and protective immunity. Here we show that the human B cell compartment in patients with diagnostically confirmed SARS-CoV-2 and clinical COVID-19 is rapidly altered with the early recruitment of B cells expressing a limited subset of IGHV genes, progressing to a highly polyclonal response of B cells with broader IGHV gene usage and extensive class switching to IgG and IgA subclasses with limited somatic hypermutation in the initial weeks of infection. We identify extensive convergence of antibody sequences across SARS-CoV-2 patients, highlighting stereotyped naive responses to this virus. Notably, sequence-based detection in COVID-19 patients of convergent B cell clonotypes previously reported in SARS-CoV infection predicts the presence of SARS-CoV/SARS-CoV-2 cross-reactive antibody titers specific for the receptor-binding domain. These findings offer molecular insights into shared features of human B cell responses to SARS-CoV-2 and other zoonotic spillover coronaviruses.
Licencia
cc_by_nc_nd
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies / Rct Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies / Rct Idioma: En Año: 2020 Tipo del documento: Preprint