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Population Bottlenecks and Intra-host Evolution during Human-to-Human Transmission of SARS-CoV-2Population Bottlenecks and Intra-host Evolution during Human-to-Human Transmission of SARS-CoV-2
Daxi Wang; Yanqun Wang; Wanying Sun; Lu Zhang; Jingkai Ji; Zhaoyong Zhang; Xinyi Cheng; Yimin Li; Fei Xiao; Airu Zhu; Jiandong Li; Peidi Ren; Zhihua Ou; Minfeng Xiao; Min Li; Ziqing Deng; Huanzi Zhong; Fuqiang Li; Weijun Chen; Shida Zhu; Wenjing Wang; Yongwei Zhang; Xun Xu; Xin Jin; Jingxian Zhao; Nanshan Zhong; Wenwei Zhang; Jincun Zhao; Junhua Li; Yonghao Xu.
Afiliación
  • Daxi Wang; BGI
  • Yanqun Wang; First Affiliated Hospital of Guangzhou Medical University
  • Wanying Sun; BGI
  • Lu Zhang; First Affiliated Hospital of Guangzhou Medical University
  • Jingkai Ji; BGI
  • Zhaoyong Zhang; First Affiliated Hospital of Guangzhou Medical University
  • Xinyi Cheng; BGI
  • Yimin Li; First Affiliated Hospital of Guangzhou Medical University
  • Fei Xiao; The Fifth Affiliated Hospital, Sun Yat-Sen University
  • Airu Zhu; First Affiliated Hospital of Guangzhou Medical University
  • Jiandong Li; BGI
  • Peidi Ren; BGI
  • Zhihua Ou; BGI
  • Minfeng Xiao; BGI
  • Min Li; BGI
  • Ziqing Deng; BGI
  • Huanzi Zhong; BGI
  • Fuqiang Li; BGI
  • Weijun Chen; BGI
  • Shida Zhu; BGI
  • Wenjing Wang; BGI
  • Yongwei Zhang; BGI
  • Xun Xu; BGI
  • Xin Jin; BGI
  • Jingxian Zhao; First Affiliated Hospital of Guangzhou Medical University
  • Nanshan Zhong; First Affiliated Hospital of Guangzhou Medical University
  • Wenwei Zhang; BGI
  • Jincun Zhao; First Affiliated Hospital of Guangzhou Medical University
  • Junhua Li; BGI
  • Yonghao Xu; First Affiliated Hospital of Guangzhou Medical University
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-173203
ABSTRACT
The emergence of the novel human coronavirus, SARS-CoV-2, causes a global COVID-19 (coronavirus disease 2019) pandemic. Here, we have characterized and compared viral populations of SARS-CoV-2 among COVID-19 patients within and across households. Our work showed an active viral replication activity in the human respiratory tract and the co-existence of genetically distinct viruses within the same host. The inter-host comparison among viral populations further revealed a narrow transmission bottleneck between patients from the same households, suggesting a dominated role of stochastic dynamics in both inter-host and intra-host evolutions. Author summaryIn this study, we compared SARS-CoV-2 populations of 13 Chinese COVID-19 patients. Those viral populations contained a considerable proportion of viral sub-genomic messenger RNAs (sgmRNA), reflecting an active viral replication activity in the respiratory tract tissues. The comparison of 66 identified intra-host variants further showed a low viral genetic distance between intra-household patients and a narrow transmission bottleneck size. Despite the co-existence of genetically distinct viruses within the same host, most intra-host minor variants were not shared between transmission pairs, suggesting a dominated role of stochastic dynamics in both inter-host and intra-host evolutions. Furthermore, the narrow bottleneck and active viral activity in the respiratory tract show that the passage of a small number of virions can cause infection. Our data have therefore delivered a key genomic resource for the SARS-CoV-2 transmission research and enhanced our understanding of the evolutionary dynamics of SARS-CoV-2.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Idioma: En Año: 2020 Tipo del documento: Preprint