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Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein
Seth J Zost; Pavlo Gilchuk; Rita Chen; James Brett Case; Joseph X Reidy; Andrew Trivette; Rachel S Nargi; Rachel E Sutton; Naveen Suryadevara; Elaine C Chen; Elad Binshtein; Swathi Shrihari; Mario A Ostrowski; Helen Y Chu; Jonathan E Didier; Keith W MacRenaris; Taylor Jones; Samuel Day; Luke Myers; F. Eun-Hyung Lee; Doan C Nguyen; Ignacio Sanz; David R Martinez; Lisa Gralinski; Ralph S Baric; Larissa Thackray; Michael S Diamond; Robert H Carnahan Jr.; James E Crowe.
Afiliación
  • Seth J Zost; Vanderbilt University Medical Center
  • Pavlo Gilchuk; Vanderbilt University Medical Center
  • Rita Chen; Washington University School of Medicine
  • James Brett Case; Washington University School of Medicine
  • Joseph X Reidy; Vanderbilt University Medical Center
  • Andrew Trivette; Vanderbilt University Medical Center
  • Rachel S Nargi; Vanderbilt University Medical Center
  • Rachel E Sutton; Vanderbilt University Medical Center
  • Naveen Suryadevara; Vanderbilt University Medical Center
  • Elaine C Chen; Vanderbilt University Medical Center
  • Elad Binshtein; Vanderbilt University Medical Center
  • Swathi Shrihari; Washington University School of Medicine
  • Mario A Ostrowski; University of Toronto
  • Helen Y Chu; University of Washington
  • Jonathan E Didier; Berkeley Lights
  • Keith W MacRenaris; Berkeley Lights
  • Taylor Jones; Vanderbilt University Medical Center
  • Samuel Day; Vanderbilt University Medical Center
  • Luke Myers; Vanderbilt University Medical Center
  • F. Eun-Hyung Lee; Emory University
  • Doan C Nguyen; Emory University
  • Ignacio Sanz; Emory University
  • David R Martinez; University of North Carolina at Chapel Hill
  • Lisa Gralinski; University of North Carolina at Chapel Hill
  • Ralph S Baric; University of North Carolina at Chapel Hill
  • Larissa Thackray; Washington University School of Medicine
  • Michael S Diamond; Washington University School of Medicine
  • Robert H Carnahan Jr.; Vanderbilt University Medical Center
  • James E Crowe; Vanderbilt University Medical Center
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-091462
Artículo de revista
Un artículo publicado en revista científica está disponible y probablemente es basado en este preprint, por medio del reconocimiento de similitud realizado por una máquina. La confirmación humana aún está pendiente.
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ABSTRACT
Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes based on their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of new antibody discovery methodologies.
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Rct Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Rct Idioma: En Año: 2020 Tipo del documento: Preprint