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Systemic analysis of tissue cells potentially vulnerable to SARS-CoV-2 infection by the protein-proofed single-cell RNA profiling of ACE2, TMPRSS2 and Furin proteases
Lulin Zhou; Zubiao Niu; Xiaoyi Jiang; Zhengrong Zhang; You Zheng; Zhongyi Wang; Yichao Zhu; Lihua Gao; Hongyan Huang; Xiaoning Wang; Qiang Sun.
Afiliación
  • Lulin Zhou; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China; School of Medicine, Nankai University, 94 Weijin Road, Tianjin, 300071, P. R. China
  • Zubiao Niu; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
  • Xiaoyi Jiang; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, 10 TI
  • Zhengrong Zhang; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
  • You Zheng; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
  • Zhongyi Wang; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
  • Yichao Zhu; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
  • Lihua Gao; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
  • Hongyan Huang; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, 10 TIEYI Road, Beijing 100038, P. R. China
  • Xiaoning Wang; School of Medicine, Nankai University, 94 Weijin Road, Tianjin, 300071, P. R. China.; School of Laboratory Medicine and Biotechnology, Southern Medical Universi
  • Qiang Sun; Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-028522
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ABSTRACT
Single-cell RNA profiling of ACE2, the SARS-CoV-2 receptor, had proposed multiple tissue cells as the potential targets of SARS-CoV-2, the novel coronavirus causing the COVID-19 pandemic. However, most were not echoed by the patients clinical manifestations, largely due to the lack of protein expression information of ACE2 and co-factors. Here, we incorporated the protein information to analyse the expression of ACE2, together with TMPRSS2 and Furin, two proteases assisting SARS-CoV-2 infection, at single cell level in situ, which we called protein-proofed single-cell RNA (pscRNA) profiling. Systemic analysis across 36 tissues revealed a rank list of candidate cells potentially vulnerable to SARS-CoV-2. The top targets are lung AT2 cells and macrophages, then cardiomyocytes and adrenal gland stromal cells, followed by stromal cells in testis, ovary and thyroid. Whereas, the polarized kidney proximal tubule cells, liver cholangiocytes and intestinal enterocytes are less likely to be the primary SARS-CoV-2 targets as ACE2 localizes at the apical region of cells, where the viruses may not readily reach. Actually, the stomach may constitute a physical barrier against SARS-CoV-2 as the acidic environment in normal stomach (pH < 2.0) could completely inactivate SARS-CoV-2 pseudo-viruses. These findings are in concert with the clinical characteristics of prominent lung symptoms, frequent heart injury, and uncommon intestinal symptoms and acute kidney injury. Together, we provide a comprehensive view on the potential SARS-CoV-2 targets by pscRNA profiling, and propose that, in addition to acute respiratory distress syndrome, attentions should also be paid to the potential injuries in cardiovascular, endocrine and reproductive systems during the treatment of COVID-19 patients.
Licencia
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2020 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Tipo de estudio: Prognostic_studies Idioma: En Año: 2020 Tipo del documento: Preprint