Blood grouping of mixed bloodstains using immunocytochemical methods.
J Forensic Sci
; 44(1): 100-4, 1999 Jan.
Article
en En
| MEDLINE
| ID: mdl-9987877
Immunocytochemical methods to determine the ABO blood group of each blood of mixed bloodstains have been developed. Mixed bloodstains were made on surgical blades and a cedar board. The blades were dipped into blood and then dipped into blood of a different group at intervals of 30, 20, 15, 10 and 5 s. Two drops of blood were dropped on a cedar board and then two drops of blood of a different group were dropped there at the same intervals. The bloodstains were dried for a week. The blood samples were removed from the blades or the cedar board and processed according with a routine histological method. Three serial thin sections were obtained. After deparaffinization, the sections were treated in papain solution for 2 h at 36 degrees C, to unmask antigenic sites on red cell membranes. The labeled streptavidin-biotin (LSAB) and peroxidase-anti-peroxidase (PAP) methods were used to detect A and B antigens, and an indirect immunocytochemical method for H antigen. These immunocytochemical methods showed specific immunologic reactions and allowed determination of the blood group of each blood of mixed bloodstains. Further, these methods indicated a possibility to determine who was stabbed first, in cases where two or more victims were stabbed with a single knife.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Manchas de Sangre
/
Sistema del Grupo Sanguíneo ABO
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Técnicas para Inmunoenzimas
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Forensic Sci
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos