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Complete sequence of p53 gene in 20 patients with lung cancer: comparison with chemosensitivity and immunohistochemistry.
Brattström, D; Bergqvist, M; Lamberg, K; Kraaz, W; Scheibenflug, L; Gustafsson, G; Inganäs, M; Wagenius, G; Brodin, O.
Afiliación
  • Brattström D; Department of Oncology, Uppsala Akademiska Hospital, Uppsala University, Sweden.
Med Oncol ; 15(4): 255-61, 1998 Dec.
Article en En | MEDLINE | ID: mdl-9951689
In this study the entire p53 complementary DNA has been sequenced in 20 non-small cell lung carcinomas (NSCLC) and the results correlated with chemosensitivity, immunohistochemistry and clinical data. Ten patients had mutations in p53, 8 missense mutations and 2 nonsense mutations. The method discovered two mutations never described previously and two other mutations that have never been described before in connection with NSCLC tumours. Chemosensitivity data, according to a short-term assay (FMCA), indicated that tumours with p53 mutation were more resistant to cisplatin and cyclophosphamide. Immunohistochemical studied demonstrated a 70% concordance between over-expression of p53 protein and mutation in p53. No conclusions or trends could be drawn from the immunohistochemical studies of Bcl-2 and Bax.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes p53 / Análisis de Secuencia de ADN / Carcinoma de Pulmón de Células no Pequeñas / Neoplasias Pulmonares / Antineoplásicos Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Med Oncol Asunto de la revista: NEOPLASIAS Año: 1998 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes p53 / Análisis de Secuencia de ADN / Carcinoma de Pulmón de Células no Pequeñas / Neoplasias Pulmonares / Antineoplásicos Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Med Oncol Asunto de la revista: NEOPLASIAS Año: 1998 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos