Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds.
Anim Genet
; 29(6): 453-5, 1998 Dec.
Article
en En
| MEDLINE
| ID: mdl-9883507
The applicability of single strand conformation polymorphism (SSCP) analysis for major histocompatibility complex (MHC) genotyping in sheep was studied. A panel of Ovar-DRB1 exon 2 'allele fingerprints' was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing sequences are used as references in SSCP analysis. In this study, seven new exon 2 sequences and 19 different alleles in total were detected from 31 animals of Finnish and Russian sheep breeds. Ovar-DRB1*0201 was detected in all the six grey Finnsheep animals included in this study, suggesting reduced MHC diversity within these animals.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ovinos
/
Polimorfismo Conformacional Retorcido-Simple
/
Antígenos de Histocompatibilidad
/
Complejo Mayor de Histocompatibilidad
Límite:
Animals
Idioma:
En
Revista:
Anim Genet
Asunto de la revista:
GENETICA
/
MEDICINA VETERINARIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Finlandia
Pais de publicación:
Reino Unido