The calcium-binding protein calretinin-22k is detectable in the serum and specific cells of cancer patients.
Anticancer Res
; 18(5B): 3661-7, 1998.
Article
en En
| MEDLINE
| ID: mdl-9854474
BACKGROUND: Calretinin-22k (CR-22k), an alternatively spliced form of calretinin (CR) belongs to the EF-hand family of calcium-binding proteins and is expressed in several colon adenocarcinoma cell lines (e.g. WiDr, HT-29). MATERIALS AND METHODS: Serum samples of cancer patients were screened with a sandwich ELISA technique using the CR-specific antiserum 7696. Highly positive samples were analyzed by Western blots and immunohistochemistry. RESULTS: CR-22k was detected in the serum of several patients and values were as high as 0.19 microgram/ml. Western blot analysis confirmed the identity of the bound protein as CR-22k. The highest concentrations were detected in patients with colon or breast cancer, but also in a patient with ischemic necrosis of the gut. CR immunoreactivity was localized to epithelial cells, nerve fibres, cells of the connective tissue and to mesothelial cells. CONCLUSIONS: Our results establish that CR-22k is detectable in the serum of cancer patients under specific pathological conditions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Proteína G de Unión al Calcio S100
/
Neoplasias del Sistema Digestivo
Límite:
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Grecia