Identification of enzymes responsible for the metabolism of heme in human platelets.
J Biol Chem
; 273(50): 33342-6, 1998 Dec 11.
Article
en En
| MEDLINE
| ID: mdl-9837908
The major enzymes involved in the degradation of heme were identified in human platelets. It was determined that heme oxygenase activity levels in umbilical cord blood platelets were higher, whereas biliverdin reductase activity levels were comparable with that found in platelets from adults. In membranes prepared from adenosine diphosphate-activated platelets, UDP-glucuronic acid-dependent bilirubin conjugation was detected, whereas activity was negligible in unactivated platelets and undetected in serum and heat-inactivated platelets, and in platelets prepared from umbilical cord blood. Platelet fractions were analyzed by Western blot and shown to express heme oxygenase, biliverdin reductase, and UDP-glucuronosyltransferases, and there was concordance with known developmental profiles found in other tissues. Heme oxygenase expression was higher, whereas UGT expression was lower, in neonatal compared with adult platelets. These data suggest that platelets are involved in multiple steps of heme and bilirubin metabolism and that developmental regulation of these enzymes may be similar to that in other human tissues.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxidorreductasas
/
Plaquetas
/
Glucuronosiltransferasa
/
Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH
/
Hemo
/
Hemo Oxigenasa (Desciclizante)
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Animals
/
Humans
/
Male
/
Newborn
Idioma:
En
Revista:
J Biol Chem
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos