Epidermal growth factor as an autocrine modulator of stress response in mammary epithelial cells.
J Endocrinol
; 159(1): 111-6, 1998 Oct.
Article
en En
| MEDLINE
| ID: mdl-9795348
Mouse mammary epithelial cells (NMuMG line) were heat shocked at 42 degreesC for up to 2 h, with continuous culture at 37 degreesC used as controls. EGF mRNA expression, determined by Northern blot hybridization, was increased 4- to 5-fold in heat-shocked cells compared with controls. Heat shock also induced accumulation of EGF immunoreactive protein in both media and cell lysates of NMuMG cells. Oligonucleotides antisense to the EGF mRNA blocked the accumulation of EGF protein, while sense controls were without effect. Antisense oligonucleotide concentrations that inhibited EGF production by NMuMG cells during heat shock dramatically reduced the ability of cells to survive heat shock, while sense oligonucleotide did not affect cell survival. The antisense oligonucleotide inhibition of cell survival was reversed by adding EGF during the heat shock period. Thus the production of EGF or EGF-like peptides during periods of cellular stress may be an important survival mechanism in mammary epithelium.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Comunicación Autocrina
/
Factor de Crecimiento Epidérmico
/
Calor
/
Glándulas Mamarias Animales
Límite:
Animals
Idioma:
En
Revista:
J Endocrinol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido