Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae.
Yeast
; 14(13): 1209-21, 1998 Sep 30.
Article
en En
| MEDLINE
| ID: mdl-9791892
Open reading frames (6116) of the budding yeast Saccharomyces cerevisiae were PCR-amplified from genomic DNA using 12,232 primers specific to the ends of the coding sequences; the success rate of amplification was 97%. PCR-products were made accessible to hybridization by being arrayed at very high density on solid support media using various robotic devices. Probes made from total RNA preparations were hybridized for the analysis of the transcriptional activity of yeast under various growth conditions and of different strains. Experimental factors that proved critical to the performance, such as different RNA isolation procedures and the assessment of hybridization results, for example, were investigated in detail. Various software tools were developed that permit convenient handling and sound analysis of the large data quantities obtained from transcriptional profiling studies. Comprehensive arrays are being distributed within the European Yeast Functional Analysis Network (EUROFAN) and beyond.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Transcripción Genética
/
Regulación Fúngica de la Expresión Génica
/
Sistemas de Lectura Abierta
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Yeast
Asunto de la revista:
MICROBIOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido