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Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: the polymerase (P3Dpol) as an immunodominant T-cell immunogen.
Collen, T; Baron, J; Childerstone, A; Corteyn, A; Doel, T R; Flint, M; Garcia-Valcarcel, M; Parkhouse, R M; Ryan, M D.
Afiliación
  • Collen T; Department of Immunology, Institute for Animal Health, Pirbright, Surrey, UK. trevor.collen@bbsrc.ac.uk
Virus Res ; 56(2): 125-33, 1998 Aug.
Article en En | MEDLINE | ID: mdl-9783461
In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3Dpol by PBMC and CD4+ T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VP1 was recognised in an homotypic context whereas VP2, VP3, VP4 and P3Dpol were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3Dpol was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3Dpol in purified virus. Thus, P3Dpol is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Cápside / Aphthovirus Límite: Animals Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 1998 Tipo del documento: Article Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Cápside / Aphthovirus Límite: Animals Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 1998 Tipo del documento: Article Pais de publicación: Países Bajos