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Infusion of GDNF into the cerebral spinal fluid through two different routes: effects on body weight and corticospinal neuron survival.
Giehl, K M; Schacht, C M; Yan, Q; Mestres, P.
Afiliación
  • Giehl KM; Department of Anatomy, University of Saarland, Homburg/Saar, Germany.
Neuroreport ; 9(12): 2809-13, 1998 Aug 24.
Article en En | MEDLINE | ID: mdl-9760125
Survival of axotomized adult rat corticospinal neurons (CSN) is supported by glial cell line-derived neurotrophic factor (GDNF). We have evaluated the trophic effects of intrathecally applied GDNF on CSN survival and rat body weight. Body weight reduction is the major side effect of intracerebral neurotrophic factor treatment. GDNF was tested at total doses of 30, 100 and 300 microg over 7 days after axotomy via different application routes: intracerebroventricularly (i.c.v.) and cisternally (cis). Animals injected i.c.v. displayed severe body weight reduction at all doses tested but CSN rescue only at the highest dose. In contrast, cis-infusion of GDNF promoted CSN survival at all doses and only the highest dose reduced the body weight. These results show that intracisternal, but not i.c.v., GDNF infusion at low doses can promote CSN survival without negatively affecting rat body weight. This finding may have implications for the clinic use of GDNF.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Peso Corporal / Corteza Cerebral / Fármacos Neuroprotectores / Factores de Crecimiento Nervioso / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: Neuroreport Asunto de la revista: NEUROLOGIA Año: 1998 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Médula Espinal / Peso Corporal / Corteza Cerebral / Fármacos Neuroprotectores / Factores de Crecimiento Nervioso / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: Neuroreport Asunto de la revista: NEUROLOGIA Año: 1998 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido