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Comparison of proteolytic enzyme activities in adults of insecticide resistant and susceptible strains of the housefly M. domestica L.
Ahmed, S; Wilkins, R M; Mantle, D.
Afiliación
  • Ahmed S; Department of Agriculture and Environmental Science, Newcastle University, Newcastle upon Tyne, UK.
Insect Biochem Mol Biol ; 28(9): 629-39, 1998 Sep.
Article en En | MEDLINE | ID: mdl-9755473
Intracellular proteolytic activity in a DDT-resistant and a susceptible strain of M. domestica was determined by assaying a comprehensive range of cytoplasmic and lysosomal enzymes. The resistant strain showed significantly higher protease activities in whole body, head, thorax, abdomen and gut homogenates compared to the susceptible strain. The activity of alanyl- and arginyl aminopeptidase from both strains increased substantially after topical treatment with DDT at 1, 2 and 3 h, suggesting an involvement of proteolytic enzymes in the induction of detoxifying enzymes, thus indicating a possible role of the intracellular proteolytic activities in the resistance mechanism.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endopeptidasas / Resistencia a los Insecticidas / DDT / Moscas Domésticas Límite: Animals Idioma: En Revista: Insect Biochem Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 1998 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endopeptidasas / Resistencia a los Insecticidas / DDT / Moscas Domésticas Límite: Animals Idioma: En Revista: Insect Biochem Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 1998 Tipo del documento: Article Pais de publicación: Reino Unido