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Design and synthesis of new potent C2-symmetric HIV-1 protease inhibitors. Use of L-mannaric acid as a peptidomimetic scaffold.
Alterman, M; Björsne, M; Mühlman, A; Classon, B; Kvarnström, I; Danielson, H; Markgren, P O; Nillroth, U; Unge, T; Hallberg, A; Samuelsson, B.
Afiliación
  • Alterman M; Department of Chemistry, Linköping University, S-581 83 Linköping, Sweden.
J Med Chem ; 41(20): 3782-92, 1998 Sep 24.
Article en En | MEDLINE | ID: mdl-9748353
A study on the use of derivatized carbohydrates as C2-symmetric HIV-1 protease inhibitors has been undertaken. L-Mannaric acid (6) was bis-O-benzylated at C-2 and C-5 and subsequently coupled with amino acids and amines to give C2-symmetric products based on C-terminal duplication. Potent HIV protease inhibitors, 28 Ki = 0.4 nM and 43 Ki = 0.2 nM, have been discovered, and two synthetic methodologies have been developed, one whereby these inhibitors can be prepared in just three chemical steps from commercially available materials. A remarkable increase in potency going from IC50 = 5000 nM (23) to IC50 = 15 nM (28) was observed upon exchanging -COOMe for -CONHMe in the inhibitor, resulting in the net addition of one hydrogen bond interaction between each of the two -NH- groups and the HIV protease backbone (Gly 48/148). The X-ray crystal structures of 43 and of 48 have been determined (Figures 5 and 6), revealing the binding mode of these inhibitors which will aid further design.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Adipatos / Proteasa del VIH / Inhibidores de la Proteasa del VIH / Imitación Molecular Límite: Animals Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 1998 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Adipatos / Proteasa del VIH / Inhibidores de la Proteasa del VIH / Imitación Molecular Límite: Animals Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 1998 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos