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Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine.
Gilman, S D; Gee, S J; Hammock, B D; Vogel, J S; Haack, K; Buchholz, B A; Freeman, S P; Wester, R C; Hui, X; Maibach, H I.
Afiliación
  • Gilman SD; Department of Entomology, University of California, Davis 95616, USA. sdgilman@utk.edu
Anal Chem ; 70(16): 3463-9, 1998 Aug 15.
Article en En | MEDLINE | ID: mdl-9726169
Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54,000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Atrazina / Conteo por Cintilación / Espectrometría de Masas / Herbicidas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Anal Chem Año: 1998 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Atrazina / Conteo por Cintilación / Espectrometría de Masas / Herbicidas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Anal Chem Año: 1998 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos