Sequential identification of apoptotic and necrotic cells on individual cytospin preparations.

Pantelidis, P; Santilli, G; Knight, R A

Pantelidis, P; Santilli, G; Knight, R A.

Afiliación

Pantelidis P; Interstitial Lung Disease Unit, Occupational and Environmental Medicine, Imperial College at the National Heart and Lung Institute, London, UK. p.pantelidis@ic.ac.uk

J Immunol Methods ; 214(1-2): 209-12, 1998 May 01.

Article en En | MEDLINE | ID: mdl-9692872

RESUMEN

Here, we describe a simple methodology which allows the consecutive differentiation of apoptotic and necrotic cells on the same cytospin preparation of fresh and cultured cells. In this methodology, necrotic cells are initially identified on the cytospin preparations using trypan blue followed by the identification of in situ DNA fragmentation using the TUNEL assay. Identification of trypan blue and TUNEL positive cells in the same section permits the simultaneous assessment of necrotic and apoptotic cells.

Asunto(s)

Apoptosis/fisiología; Técnicas Citológicas; Necrosis; Líquido del Lavado Bronquioalveolar/citología; ADN/análisis; ADN/metabolismo; ADN de Neoplasias/análisis; ADN de Neoplasias/metabolismo; Células HL-60/citología; Células HL-60/patología; Humanos; Azul de Tripano

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Citológicas / Apoptosis / Necrosis Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Immunol Methods Año: 1998 Tipo del documento: Article Pais de publicación: Países Bajos

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