Solution structure of a zinc domain conserved in yeast copper-regulated transcription factors.

Turner, R B; Smith, D L; Zawrotny, M E; Summers, M F; Posewitz, M C; Winge, D R

Turner, R B; Smith, D L; Zawrotny, M E; Summers, M F; Posewitz, M C; Winge, D R.

Afiliación

Turner RB; Howard HUghes Medical Institute, University of Maryland Baltimore County 21250, USA.

Nat Struct Biol ; 5(7): 551-5, 1998 Jul.

Article en En | MEDLINE | ID: mdl-9665167

RESUMEN

The three dimensional structure of the N-terminal domain (residues 1-42) of the copper-responsive transcription factor Amtl from Candida glabrata has been determined by two-dimensional 1H-correlated nuclear magnetic resonance (NMR) methods. The domain contains an array of zinc-binding residues (Cys-X2-Cys-X8-Cys-X-His) that is conserved among a family of Cu-responsive transcription factors. The structure is unlike those of previously characterized zinc finger motifs, and consists of a three-stranded antiparallel beta-sheet with two short helical segments that project from one end of the beta-sheet. Conserved residues at positions 16, 18 and 19 form a basic patch that may be important for DNA binding.

Asunto(s)

Candida/química; Proteínas de Unión al ADN/química; Estructura Secundaria de Proteína; Factores de Transcripción/química; Zinc/química; Secuencia de Aminoácidos; Cobre/farmacología; Cisteína/química; Proteínas Fúngicas; Datos de Secuencia Molecular; Resonancia Magnética Nuclear Biomolecular

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Zinc / Candida / Estructura Secundaria de Proteína / Proteínas de Unión al ADN Idioma: En Revista: Nat Struct Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 1998 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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