[Development of the technology of the purification of Coxiella burnetii from components of culture medium]. / Razrabotka tekhnologii ochistki Coxiella burnetii ot komponentov sredy kyl'tivirovaniia.
Zh Mikrobiol Epidemiol Immunobiol
; (2): 59-62, 1998.
Article
en Ru
| MEDLINE
| ID: mdl-9662802
The technology of the purification of C.burnetii from the yolk-sac components of the cultivation medium, including differential centrifugation, membrane filtration, enzymatic hydrolysis, the standardization of the preparation thus obtained and its stabilization by the method of lyophilization, was developed. Antigens, prepared in accordance with this technology, were sufficiently active and highly specific. The possibility of their use in different serological tests (the complement fixation test and the indirect fluorescent analysis) was studied. In addition, these antigens were found to have good capacity for sorption on the solid-phase carrier (plates) and to be suitable for use in such highly sensitive test as the enzyme immunoassay.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Coxiella burnetii
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Ru
Revista:
Zh Mikrobiol Epidemiol Immunobiol
Año:
1998
Tipo del documento:
Article
Pais de publicación:
Rusia