Colon-specific genotoxicity of heterocyclic amines detected by the modified alkaline single cell gel electrophoresis assay of multiple mouse organs.
Mutat Res
; 414(1-3): 9-14, 1998 May 11.
Article
en En
| MEDLINE
| ID: mdl-9630478
The in vivo genotoxicity of five heterocyclic amines-Trp-P-2 (13 mg/kg), IQ (13 mg/kg), MeIQ (13 mg/kg), MeIQx (13 mg/kg), and PhIP (40 mg/kg)-in the mucosa of gastrointestinal and urinary tract organs (stomach, duodenum, jejunum, ileum, colon, and bladder) was studied by the alkaline single cell gel electrophoresis (SCG) (Comet) assay. Male CD-1 mice were sacrificed 1, 3, and 8 h after intraperitoneal injection. All the heterocyclic amines studied yielded statistically significant DNA damage in the colon but not the small intestine (duodenum, jejunum, and ileum) or urinary bladder. In this study, five heterocyclic amines were injected intraperitoneally to avoid the consequences of ingestion. Thus, the extensive damage to colon DNA was concluded to be due, at least in part, to a systemic effect.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colon
/
Mucosa Gástrica
/
Aminas
/
Mucosa Intestinal
/
Pruebas de Mutagenicidad
/
Mutágenos
Límite:
Animals
Idioma:
En
Revista:
Mutat Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos