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Expression of an N-methyl-D-aspartate-type receptor by human and rat osteoblasts and osteoclasts suggests a novel glutamate signaling pathway in bone.
Patton, A J; Genever, P G; Birch, M A; Suva, L J; Skerry, T M.
Afiliación
  • Patton AJ; Department of Biology, University of York, United Kingdom. ajp12@york.ac.uk
Bone ; 22(6): 645-9, 1998 Jun.
Article en En | MEDLINE | ID: mdl-9626403
Signaling between the various types of cells found in bone is responsible for controlling the activity of osteoblasts and osteoclasts, and therefore the regulation of bone mass. Our identification of a neuronal glutamate transporter in osteoblasts and osteocytes suggests the possibility that bone cells may use the excitatory amino acid glutamate as a signaling molecule. In these studies we report the expression of different subtypes of glutamate receptors in osteoblasts and osteoclasts in vitro and in vivo. We have identified expression in human and rat bone cells of N-methyl-D-aspartate receptor-1 (NMDAR-1) and 2D subunits and PSD-95, the NMDA receptor clustering protein associated with signaling in the central nervous system. In situ hybridization and immunohistochemistry localized NMDAR-1 expression to osteoblasts and osteoclasts in human tissue sections. These findings strengthen the suggestion that glutamate is involved in signaling between bone cells.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Osteoclastos / Receptores de N-Metil-D-Aspartato / Ácido Glutámico / Transportadoras de Casetes de Unión a ATP Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Bone Asunto de la revista: METABOLISMO / ORTOPEDIA Año: 1998 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Osteoclastos / Receptores de N-Metil-D-Aspartato / Ácido Glutámico / Transportadoras de Casetes de Unión a ATP Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Bone Asunto de la revista: METABOLISMO / ORTOPEDIA Año: 1998 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos