Direct detection of domains in phospholipid bilayers by grazing incidence diffraction of neutrons and atomic force microscopy.
Biophys J
; 74(5): 2443-50, 1998 May.
Article
en En
| MEDLINE
| ID: mdl-9591670
The geometry of domains in phospholipid bilayers of binary (1:1) mixtures of synthetic lecithins with a difference in chain length of four methylene groups has been studied by two independent, direct and complementary methods. Grazing incidence diffraction of neutrons provided gel domain sizes of less than 10 nm in both the gel and the coexistence phase of the mixture, while no domains were detected for the fluid phase. For the coexistence region, the neutron data suggest that domains grow in number rather than in size with decreasing temperature. Atomic force microscopy was used to study gel phase size and shape of the domains. The domains imaged by atomic force microscopy exhibit a rather irregular shape with an average size of 10 nm, thus confirming the neutron results for this phase. The good agreement between atomic force microscopy and neutron results, despite the completely different nature of their observables, has potential for the future development of refined models for the interpretation of neutron data from heterogeneous membranes in terms of regularly spaced and spatially extended scatterers.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Membrana Dobles de Lípidos
Tipo de estudio:
Diagnostic_studies
/
Incidence_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Biophys J
Año:
1998
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos