Nonvagal origin of galanin-containing nerve terminals innervating striated muscle fibers of the rat esophagus.
Cell Tissue Res
; 292(3): 453-61, 1998 Jun.
Article
en En
| MEDLINE
| ID: mdl-9582402
We investigated the origin of galanin-positive nerve fibers on motor endplates in rat esophagus using anterograde 1,1'-dioleyl-3,3,3', 3'-tetramethylindocarbocyanine methane sulfonate (DiI) tracing from the nucleus ambiguus combined with galanin immunocytochemistry and calcitonin gene-related peptide immunocytochemistry. To demonstrate spatial relationships of galanin-positive nerve fibers to vagal and enteric nerve fibers on motor endplates, we combined galanin immunocytochemistry with calcitonin gene-related peptide immunostaining for labeling of vagal terminals, and vasoactive intestinal peptide immunoreactivity and NADPH-diaphorase histochemistry for demonstration of enteric nerve fibers. Within fine varicose nerve fibers, galanin was colocalized with vasoactive intestinal peptide and NADPH-diaphorase to a high degree and turned out to be completely separated from calcitonin gene-related peptide-positive or anterogradely DiI-labeled vagal motor terminals. These results indicate that the enteric nervous system is the most important and possibly the only source of galanin-positive nerve terminals on motor endplates in rat esophagus. Galanin may be, in addition to nitric oxide and vasoactive intestinal peptide, a mediator of the enteric coinnervation of striated muscle in this organ.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nervio Vago
/
Músculo Esquelético
/
Galanina
/
Esófago
/
Terminaciones Nerviosas
Límite:
Animals
Idioma:
En
Revista:
Cell Tissue Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Alemania