Synthesis and hybridization properties of an acyclic achiral phosphonate DNA analogue.
Bioorg Med Chem
; 6(3): 315-22, 1998 Mar.
Article
en En
| MEDLINE
| ID: mdl-9568285
Protected N-(2-hydroxyethyl)-N-(nucleobase-acetyl)aminomethanephosphonic+ ++ acid (6a-d) of all four DNA nucleobases have been prepared and oligomerized by solid-phase synthesis. Four DNA decamers containing 1-10 of these 'PPNA' monomers were prepared and evaluated by Tm measurements (medium salt) for binding to their DNA and RNA complements. One central modification reduced the binding strongly (delta Tm = -10 degrees C), but contiguous PPNA monomers gave smaller effects, and the all-PPNA decamer bound to RNA with a delta Tm of -1.2 degrees C per modification. Thus PPNA oligomers are inferior DNA and RNA binders compared to the closely related and strongly binding PNA oligomers.
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01-internacional
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MEDLINE
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En
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Bioorg Med Chem
Asunto de la revista:
BIOQUIMICA
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QUIMICA
Año:
1998
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Article
País de afiliación:
Dinamarca
Pais de publicación:
Reino Unido