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IL-12 prevents neonatal induction of transplantation tolerance in mice.
Donckier, V; Flamand, V; Desalle, F; Vanderhaeghen, M L; de Veerman, M; Thielemans, K; Abramowicz, D; Goldman, M.
Afiliación
  • Donckier V; Laboratory of Experimental Immunology, Université Libre des Bruxelles, Brussels, Belgium.
Eur J Immunol ; 28(4): 1426-30, 1998 Apr.
Article en En | MEDLINE | ID: mdl-9565383
We investigated the effect of IL-12 on the induction of transplantation tolerance by neonatal injection of allogenic cells. We first observed that injection of newborn BALB/c mice with IL-12 and (A/J x BALB/c)F1 spleen cells prevented the Th2 alloimmune response induced by neonatal inoculation of F1 cells alone and allowed the differentiation of T cells secreting high amounts of IL-2 and IFN-gamma in mixed lymphocyte cultures with donor-type stimulators. Furthermore, IL-12 administration resulted in the emergence of anti-donor cytotoxic T lymphocyte responses although at lower levels than in control uninjected mice. In parallel, we found that mice injected at birth with IL-12 and F1 cells did not develop chimerism and were able to reject a donor-type skin graft as efficiently as control mice. We conclude that IL-12 inhibits the Th2 polarization of the newborn response to alloantigens and prevents thereby the establishment of transplantation tolerance.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Células / Células Th2 / Interleucina-12 / Supervivencia de Injerto / Tolerancia Inmunológica Límite: Animals Idioma: En Revista: Eur J Immunol Año: 1998 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Células / Células Th2 / Interleucina-12 / Supervivencia de Injerto / Tolerancia Inmunológica Límite: Animals Idioma: En Revista: Eur J Immunol Año: 1998 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Alemania