Identification of a putative receptor for porcine reproductive and respiratory syndrome virus on porcine alveolar macrophages.
J Virol
; 72(5): 4520-3, 1998 May.
Article
en En
| MEDLINE
| ID: mdl-9557752
To identify the receptor which may determine the macrophage tropism of porcine reproductive and respiratory syndrome virus (PRRSV), monoclonal antibodies (MAbs) against porcine alveolar macrophages (PAM) were produced. Two MAbs (41D3 and 41D5) which completely blocked PRRSV infection of PAM were further characterized. It was found that they reduce the attachment of PRRSV to PAM and immunoprecipitate a 210-kDa membrane protein from PAM. This protein was detected on the cell membranes of PAM but not of PRRSV-nonpermissive cells. A colocalization was found between the reactive sites of MAb 41D3 and PRRSV on PAM membranes. All PRRSV-infected cells in tissues of experimentally infected pigs reacted with MAb 41D3. Taken together, all these data suggest that the identified 210-kDa membrane protein is a putative receptor for PRRSV on porcine macrophages.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores Virales
/
Macrófagos Alveolares
/
Virus del Síndrome Respiratorio y Reproductivo Porcino
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Virol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Bélgica
Pais de publicación:
Estados Unidos