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Indirect assessment of 4,4'-diphenylmethane diisocyanate (MDI) exposure by evaluation of specific humoral immune responses to MDI conjugated to human serum albumin.
Lushniak, B D; Reh, C M; Bernstein, D I; Gallagher, J S.
Afiliación
  • Lushniak BD; Hazard Evaluations and Technical Assistance Branch, National Institute for Occupational Safety and Health, Cincinnati, OH, USA.
Am J Ind Med ; 33(5): 471-7, 1998 May.
Article en En | MEDLINE | ID: mdl-9557170
BACKGROUND: A study of occupational asthma among workers exposed to 4,4'-Diphenylmethane Diisocyanate (MDI). OBJECTIVE: To demonstrate if serum concentrations of MDI-specific IgG or IgE are sensitive biological markers of disease or of MDI exposure. METHODS: The study group consisted of nine MDI-exposed workers and nine nonexposed workers. Air sampling for MDI and polymethylene polyphenyl isocyanate, occupational and medical histories, respiratory physical exams, pre- and postshift spirometry, and self-administered peak expiratory flow rates were performed. Serum specific IgE and IgG antibodies to an MDI-human serum albumin (HSA) conjugate were assayed by the radioallergosorbent test and the enzyme-linked immunosorbent assay, respectively, and compared to nine nonexposed laboratory controls. RESULTS: No definitive cases of occupational asthma were documented. The mean level of MDI-specific IgG was significantly greater among exposed workers compared to nonexposed workers and laboratory controls (p = 0.04). Mean levels of TDI and HDI-specific IgG were also increased. CONCLUSION: This study demonstrates that serum concentrations of MDI-specific IgG appear to be a moderately sensitive biological marker of MDI exposure, but not an indicator of occupational asthma. Workers with IgG antibodies specific for one diisocyanate-HSA conjugate exhibit cross-reactivity to antigens prepared with other diisocyanates.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alérgenos / Exposición Profesional / Isocianatos Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male Idioma: En Revista: Am J Ind Med Año: 1998 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alérgenos / Exposición Profesional / Isocianatos Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male Idioma: En Revista: Am J Ind Med Año: 1998 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos