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Detection of Eimeria acervulina using the polymerase chain reaction.
Molloy, J B; Eaves, F W; Jeston, P J; Minchin, C M; Stewart, N P; Lew, A E; Jorgensen, W K.
Afiliación
  • Molloy JB; Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
Avian Dis ; 42(1): 119-23, 1998.
Article en En | MEDLINE | ID: mdl-9533088
A polymerase chain reaction (PCR)-based assay was developed for the detection of Eimeria acervulina. Primers were designed to amplify a fragment of the EASZ240/160 sporozoite antigen gene. The PCR assay detected as few as 10 E. acervulina oocysts in a mixed population containing a total of 10(6) oocysts. No nonspecific reaction was observed with any other species of avian Eimeria known to occur in Australia. PCR products from genomic DNA were 237 bp larger than predicted from previously reported cDNA sequences. Sequencing of the product revealed the presence of a probable intron. This work demonstrates the potential of PCR-based assays for identification and detection of avian Eimeria. Potential uses include identification of minor species present in mixed infections and quality control in the production of live vaccines.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Reacción en Cadena de la Polimerasa / Coccidiosis / Eimeria Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: Avian Dis Año: 1998 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Reacción en Cadena de la Polimerasa / Coccidiosis / Eimeria Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: Avian Dis Año: 1998 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos