Genomic DNA sequence of Rhesus (M. mulatta) cystic fibrosis (CFTR) gene.
Mamm Genome
; 9(4): 301-5, 1998 Apr.
Article
en En
| MEDLINE
| ID: mdl-9530627
Cystic fibrosis is a common human genetic disease caused by mutations in CFTR, a gene that codes for a chloride channel that is regulated by phosphorylation and cytosolic nucleotides. As part of a program to discover natural animal models for human genetic diseases, we have determined the genomic sequence of CFTR in the Rhesus monkey, Macaca mulatta. The coding region of rhesus CFTR is 98.3% identical to human CFTR at the nucleotide level and 98.2% identical and 99.7% similar at the amino acid level. Partial sequences of flanking introns (5582 base pair positions analyzed) revealed 91.1% identity with human introns. Relative to rhesus intronic sequence, the human sequences had 27 insertions and 22 deletions. Primer sequences for amplification of rhesus genomic CFTR sequences are provided. The accession number is AF013753 (all 27 exons and some flanking intronic sequence).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Regulador de Conductancia de Transmembrana de Fibrosis Quística
/
Macaca mulatta
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Mamm Genome
Asunto de la revista:
GENETICA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos