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Separation of proteolytic enzymes originating from Antarctic krill (Euphausia superba) by capillary electrophoresis.
Sjödahl, J; Emmer, A; Karlstam, B; Vincent, J; Roeraade, J.
Afiliación
  • Sjödahl J; Royal Institute of Technology, Dept of Analytical Chemistry, Stockholm, Sweden.
J Chromatogr B Biomed Sci Appl ; 705(2): 231-41, 1998 Feb 13.
Article en En | MEDLINE | ID: mdl-9521559
Extracts prepared from Antarctic krill (Euphausia superba), mainly consisting of acidic proteolytic enzymes, have been studied with capillary electrophoretic techniques. Approximately 50 repeatable peaks were obtained with capillary zone electrophoresis on an untreated fused-silica capillary using a phosphate buffer containing anionic and cationic fluorosurfactant additives as separation medium. A faster separation was achieved on a polyvinyl alcohol coated capillary. Quantitative variations of individual proteins regarding different krill enzyme batches were noted. In the krill samples trypsin-like serine proteinase, carboxypeptidase A and carboxypeptidase B were tentatively identified.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Electroforesis Capilar Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Chromatogr B Biomed Sci Appl Asunto de la revista: QUIMICA CLINICA Año: 1998 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Electroforesis Capilar Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Chromatogr B Biomed Sci Appl Asunto de la revista: QUIMICA CLINICA Año: 1998 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Países Bajos