Molecular beacons: a new approach for semiautomated mutation analysis.
Clin Chem
; 44(3): 482-6, 1998 Mar.
Article
en En
| MEDLINE
| ID: mdl-9510851
Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We designed molecular beacons to detect a point mutation in the methylenetetrahydrofolate reductase (MTHFR) gene, a mutation that has been related to an increased risk for cardiovascular disease and neural tube defects. The application of molecular beacons enables fast, semiautomated, accurate mutation detection. Moreover, the procedure is performed in a closed tube system, thereby avoiding carryover contamination. We believe these probes will find their way into nucleic acid research and diagnostics.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Análisis Mutacional de ADN
/
Sondas de Oligonucleótidos
/
Mutación Puntual
/
Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH
Tipo de estudio:
Etiology_studies
/
Risk_factors_studies
Límite:
Child
/
Humans
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Reino Unido