Erythrocyte calcium metabolism. Calcium exchange in normal and sickle-cell-anaemia erythrocytes.
Biochem J
; 156(3): 577-83, 1976 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-949340
Under exchange conditions (no net increase in calcium), erythrocytes incubated in isoosmotic phosphate-buffered saline have an exchangeable calcium pool comprising about 10% of the total erythrocyte calcium. This pool reaches exchange equilibrium, for either inward-directed or outward-directed transfer of the 45Ca-exchange label, with a half-time of about 20 min. The uptake of Ca2+ requires phosphate, even under hypo-osmotic conditions, where the calcium loading expected as the cells swell is obtained only when phosphate is present. The phosphate requirement is not due to Ca2+ transport as a phosphate salt. This exchangeable-calcium pool is also present in sickle-cell-anemia erythrocytes, and comprises a similar proportion of total cellular calcium.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Calcio
/
Eritrocitos
/
Anemia de Células Falciformes
Límite:
Humans
Idioma:
En
Revista:
Biochem J
Año:
1976
Tipo del documento:
Article
Pais de publicación:
Reino Unido