Can monomers of yeast enolase have enzymatic activity?
Eur J Biochem
; 251(3): 775-80, 1998 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-9490051
Using a combination of ultraviolet spectroscopy under pressure and stopped-flow kinetics under pressure, we have shown that the monomers of yeast enolase produced by hydrostatic pressure are inactive. K(eq), deltaV and deltaV for the dissociation/inactivation produced by hydrostatic pressure have been determined under various conditions. Removing the Mg2+ from enolase, either by adding EDTA or by preparing apoenzyme, displaces the equilibrium towards monomers and decreases both deltaV and deltaV. Loss of Mg2+ contributes to the negative deltaV for dissociation; this loss occurs, at least partially, in the transition state for dissociation. Both removal of Mg(II) and dissociation of the enzyme produce major changes in the intensity of the aromatic region of the CD spectrum. We propose that these changes in the CD spectra reflect changes in the conformations of the 'mobile loops' of enolase. The precise conformation of these, loops is necessary for binding Mg2+ (and, hence, for activity) and for maintaining subunit interactions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfopiruvato Hidratasa
/
Conformación Proteica
/
Saccharomyces cerevisiae
Idioma:
En
Revista:
Eur J Biochem
Año:
1998
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Reino Unido