The C terminus of the major yeast telomere binding protein Rap1p enhances telomere formation.
Mol Cell Biol
; 18(3): 1284-95, 1998 Mar.
Article
en En
| MEDLINE
| ID: mdl-9488443
The telomeres of most organisms consist of short repeated sequences that can be elongated by telomerase, a reverse transcriptase complex that contains its own RNA template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the RAP1 gene encodes the major telomere binding protein Rap1p. Here we use a quantitative telomere formation assay to demonstrate that Rap1p C termini can enhance telomere formation more than 30-fold when they are located at internal sites. This stimulation is distinct from protection from degradation. Enhancement of formation required the gene for telomerase RNA but not Sir1p, Sir2p, Sir3p, Sir4p, Tel1p, or the Rif1p binding site in the Raplp C terminus. Our data suggest that Rap1p C termini enhance telomere formation by attracting or increasing the activity of telomerase near telomeres. Earlier work suggests that Rap1p molecules at the chromosome terminus inhibit the elongation of long telomeres by blocking the access of telomerase. Our results suggest a model where a balance between internal Rap1p increasing telomerase activity and Rap1p at the termini of long telomeres controlling telomerase access maintains telomeres at a constant length.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Factores de Transcripción
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Telómero
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Unión a Telómeros
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Proteínas Reguladoras de Información Silente de Saccharomyces cerevisiae
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Proteínas de Unión al ADN
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Histona Desacetilasas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Mol Cell Biol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos