Rat brain contains multiple mRNAs for calpastatin.

De Tullio, R; Sparatore, B; Salamino, F; Melloni, E; Pontremoli, S

De Tullio, R; Sparatore, B; Salamino, F; Melloni, E; Pontremoli, S.

Afiliación

De Tullio R; Institute of Biological Chemistry, University of Genoa, Italy.

FEBS Lett ; 422(1): 113-7, 1998 Jan 23.

Article en En | MEDLINE | ID: mdl-9475181

RESUMEN

This work was undertaken to establish the forms of the calpain inhibitor, calpastatin, expressed in the brain tissue. Five cDNA clones were obtained and the corresponding amino acid sequences were deduced. Three of these proteins contain an N-terminal domain (domain L) and four inhibitory repeats typical of the calpastatin molecule. The other two are truncated forms, containing the domain L, free or associated with a single inhibitory repeat. Other differences, due to exon skipping, produce calpastatin forms with different susceptibility to posttranslational modifications. The more represented mRNA form corresponds to a calpastatin molecule containing the four inhibitory domains. These results may be useful to understand the involvement of calpain in the onset of acute and degenerative disorders of the central nervous system.

Asunto(s)

Encéfalo/metabolismo; Proteínas de Unión al Calcio/química; ARN Mensajero/genética; Secuencia de Aminoácidos; Animales; Enfermedades del Sistema Nervioso Central/fisiopatología; Clonación Molecular; Hígado/química; Datos de Secuencia Molecular; Procesamiento Proteico-Postraduccional/genética; ARN Mensajero/metabolismo; Ratas; Alineación de Secuencia; Análisis de Secuencia de ADN; Inhibidores de Serina Proteinasa

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Proteínas de Unión al Calcio / ARN Mensajero Límite: Animals Idioma: En Revista: FEBS Lett Año: 1998 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

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