[Nucleic acids interactions with short oligonucleotide derivatives. II. Tandem of short oligonucleotides as highly sensitive system for identification of single base substitutions in target DNA]. / Vzaimodeistvie proizvodnykh korotkikh oligonukleotidov s nykleinovymi kislotami. II. Tandem korotkikh oligonukleotidov--vysokochuvstvitel'naia sistema dlia obnaruzheniia odnobukvennoi zameny v DNK-misheni.
Bioorg Khim
; 23(7): 561-8, 1997 Jul.
Article
en Ru
| MEDLINE
| ID: mdl-9471976
A new approach for modification of target DNAs with tandems of derivatives of short oligonucleotides was suggested that allows highly selective modification of perfect duplexes only. At physiological temperatures, the efficiency of DNA modification by a dodecanucleotide alkylating agent was demonstrated to be the same for both perfect and mismatch-containing duplexes, whereas the tetranucleotide reagent in the presence of two flanking effectors alkylated with high selectivity the target DNA in the perfect duplex only.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligonucleótidos
/
ADN
/
Mutación Puntual
/
Alquilantes
Tipo de estudio:
Diagnostic_studies
Idioma:
Ru
Revista:
Bioorg Khim
Año:
1997
Tipo del documento:
Article
Pais de publicación:
Rusia