Free radical production after exposure of astrocytes and astrocytic C6 glioma cells to ethanol. Preliminary results.
Free Radic Res
; 27(6): 645-56, 1997 Dec.
Article
en En
| MEDLINE
| ID: mdl-9455700
Formation of the alpha-hydroxyethyl radical (CH3 degree CHOH) has already been extensively demonstrated after ethanol metabolism in the liver. Despite favourable conditions, this formation in the brain has remained speculative since there is no direct experimental evidence in intact brain cells. In this preliminary study, the formation of such a radical was observed after exposure of astrocytes and astrocytic C6 glioma cells to ethanol. These cells were studied because astrocyte integrity is essential for normal growth and functioning of neurons. The free radicals were detected by EPR spectroscopy using the spin trapping technique. Astrocytes appeared to be more sensitive than the C6 cells to free radical formation as the intensity of the signal was higher after exposure of the astrocytes and increased with time, a fact not observed after exposure of the C6 cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Astrocitoma
/
Neoplasias Encefálicas
/
Astrocitos
/
Etanol
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Free Radic Res
Asunto de la revista:
BIOQUIMICA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido