Preparation of a 'beheaded' derivative of the 30S ribosomal subunit.

Ulitin, A B; Agalarov, S Ch; Serdyuk, I N

Ulitin, A B; Agalarov, S Ch; Serdyuk, I N.

Afiliación

Ulitin AB; Institute of Protein Research, Russian Academy of Sciences, Moscow Region, Russia.

Biochimie ; 79(8): 523-6, 1997 Sep.

Article en En | MEDLINE | ID: mdl-9451454

RESUMEN

Oligodesoxyribonucleotide-directed cleavage of protein-deficient Thermus thermophilus derivatives of the 30S ribosomal subunits with RNase H is described. A homogeneous RNP fragment has been isolated as a result of the cleavage and subsequent purification in the sucrose gradient. It corresponds to the central and 5' domains of the 30S ribosomal subunit. The high compactness of the fragment in solution suggests that it can be considered as a 'beheaded' derivative of the 30S ribosomal subunit. The absence of a reconstitution stage in isolation of the 22S RNP fragment provides for its preparation in large amounts.

Asunto(s)

ARN Ribosómico 16S/química; Ribonucleoproteínas/aislamiento & purificación; Proteínas Ribosómicas/análisis; Ribosomas/fisiología; Thermus thermophilus/genética; Centrifugación por Gradiente de Densidad; Electroforesis en Gel Bidimensional; Ribonucleasas/metabolismo; Ribonucleoproteínas/química; Thermus thermophilus/química

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleoproteínas / Proteínas Ribosómicas / Ribosomas / ARN Ribosómico 16S / Thermus thermophilus Idioma: En Revista: Biochimie Año: 1997 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Francia

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