Evaluation of a new method for identification of bacteria based on sequence homology of 16S rRNA gene.
PDA J Pharm Sci Technol
; 51(6): 242-7, 1997.
Article
en En
| MEDLINE
| ID: mdl-9448434
A new identification method for bacteria based on partial sequences of divergent regions of the 16S rRNA gene was evaluated. The method involves PCR-based amplification of 16S rRNA gene fragments, followed by sequencing and comparison of sequences of about 300 nucleotides with those in the database of NCBI (National Center for Biotechnology Information) via the Internet. Most of the bacteria tested could be identified at the species level even if some unread nucleotides were present in the sequence. Although this method still requires improvement, it has the potential to be a highly reliable and practical identification method for bacteria.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
ADN Ribosómico
/
ARN Ribosómico 16S
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
PDA J Pharm Sci Technol
Asunto de la revista:
FARMACIA
/
FARMACOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos