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Evaluation of a new method for identification of bacteria based on sequence homology of 16S rRNA gene.
Sasaki, T; Nishiyama, T; Shintani, M; Kenri, T.
Afiliación
  • Sasaki T; National Institute of Infectious Diseases, Tokyo, Japan.
PDA J Pharm Sci Technol ; 51(6): 242-7, 1997.
Article en En | MEDLINE | ID: mdl-9448434
A new identification method for bacteria based on partial sequences of divergent regions of the 16S rRNA gene was evaluated. The method involves PCR-based amplification of 16S rRNA gene fragments, followed by sequencing and comparison of sequences of about 300 nucleotides with those in the database of NCBI (National Center for Biotechnology Information) via the Internet. Most of the bacteria tested could be identified at the species level even if some unread nucleotides were present in the sequence. Although this method still requires improvement, it has the potential to be a highly reliable and practical identification method for bacteria.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / ADN Ribosómico / ARN Ribosómico 16S Tipo de estudio: Diagnostic_studies Idioma: En Revista: PDA J Pharm Sci Technol Asunto de la revista: FARMACIA / FARMACOLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / ADN Ribosómico / ARN Ribosómico 16S Tipo de estudio: Diagnostic_studies Idioma: En Revista: PDA J Pharm Sci Technol Asunto de la revista: FARMACIA / FARMACOLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos