Quality control of Kato slide counts for Schistosoma mansoni: a review of 12 years' experience in Kenya.
Bull World Health Organ
; 75(5): 469-75, 1997.
Article
en En
| MEDLINE
| ID: mdl-9447781
ABSTRACT
PIP: When Kato slides are stored properly, the number of Schistosoma mansoni eggs in fecal smears remains countable for many months after preparation--a feature facilitating quality control studies in parasite control programs with limited resources. The present study compared egg recounts performed by independent microscopists in a total of 10,113 slides obtained in 19 annual or biannual audits with the original counts made by Division of Vector-borne Diseases (DVBD) microscopists in Kenya's Machakos and Makueni Districts in 1984-96. Recounts were performed 1-18 months after initial slide preparation. The overall proportion of discrepant counts in the 12-year study period was 6.83%. The majority of discrepant counts involved light infestations (50 eggs/g). At each audit, more slides were recorded as positive by DVBD microscopists and negative by the auditor than were recorded as negative by the DVBD and positive by the auditor. This trend is presumed to reflect Kato slide deterioration--especially a drying out before storage in hot, dry weather--between the initial count and the audit. Mean DVBD egg counts declined steadily between audits 10 (1989) and 19 (1996) in tandem with intensified treatment campaigns in the area. These findings confirm the suitability of this technique for quality control in programs with limited funds.
Palabras clave
Africa; Africa South Of The Sahara; Clinical Research; Developing Countries; Diseases; Eastern Africa; English Speaking Africa; Examinations And Diagnoses; Health; Kenya; Laboratory Examinations And Diagnoses; Organization And Administration; Parasite Control; Parasitic Diseases; Public Health; Quality Control; Research Methodology; Research Report
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recuento de Huevos de Parásitos
/
Schistosoma mansoni
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
Bull World Health Organ
Año:
1997
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Suiza