T cell populations and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands.

Taylor, B C; Keefe, R G; Dellinger, J D; Nakamura, Y; Cullor, J S; Stott, J L

Taylor, B C; Keefe, R G; Dellinger, J D; Nakamura, Y; Cullor, J S; Stott, J L.

Afiliación

Taylor BC; Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA. bctaylor@mail.uwlax.edu

Cell Immunol ; 182(1): 68-76, 1997 Nov 25.

Article en En | MEDLINE | ID: mdl-9427811

RESUMEN

This study investigated the effect of naturally acquired bacterial infection of the bovine mammary gland on subpopulations of T lymphocytes and cytokine expression in milk. Twenty-nine lactating cows with mastitis were compared to 12 normal animals. CD4+ lymphocytes represented a significantly greater percentage of the milk-derived lymphocytes in infected mammary glands compared to normal controls. Cytokine mRNA expression by cells derived from milk was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR). No IL-2 or IL-4 mRNA was detected in any samples, while IFN-gamma mRNA was detected in all milk samples. IL-10 mRNA was detected in cells from the milk of 2 mastitic cows and 1 normal cow, and IL-12 mRNA was detected in 2 cows with mastitis. While TNF-alpha mRNA was not detected in this study, IL-6 mRNA was identified in cells from the milk of all animals, with levels being greater in mastitic animals.

Asunto(s)

Infecciones Bacterianas/veterinaria; Citocinas/genética; Mastitis Bovina/inmunología; Leche/inmunología; Subgrupos de Linfocitos T/inmunología; Animales; Infecciones Bacterianas/inmunología; Infecciones Bacterianas/prevención & control; Secuencia de Bases; Relación CD4-CD8; Bovinos; Cartilla de ADN/genética; Femenino; Expresión Génica; Lactancia/inmunología; Recuento de Leucocitos; Mastitis Bovina/prevención & control; Leche/citología; Reacción en Cadena de la Polimerasa; ARN Mensajero/genética; ARN Mensajero/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Bacterianas / Citocinas / Subgrupos de Linfocitos T / Leche / Mastitis Bovina Límite: Animals Idioma: En Revista: Cell Immunol Año: 1997 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos

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