GEMHEP multicenter quality control study of PCR detection of GB virus C/hepatitis G virus RNA in serum.
J Clin Microbiol
; 35(12): 3298-300, 1997 Dec.
Article
en En
| MEDLINE
| ID: mdl-9399538
PCR is, to date, the only available tool for the detection of GB virus C (GBV-C) and hepatitis G virus (HGV) RNAs. Twenty-two French laboratories participated in a quality control study to assess the sensitivity and specificity of their procedures. The panel included 13 positive controls and 7 negative controls. The laboratories used either in-house PCR techniques adapted from the literature or partly standardized commercial tests. Three laboratories performed faultlessly with the entire panel. Most laboratories had excellent specificity (100% in 20 of 22 laboratories). Sensitivity was acceptable (85 to 100%) in 15 centers and insufficient (38 to 77%) in 7. As with nonstandardized in-house PCR, the commercial assays gave discrepant performances in different laboratories. These results suggest that laboratories willing to use PCR for detection of GBV-C/HGV RNA for research or diagnostic purposes should participate in multicenter quality control trials.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virología
/
ARN Viral
/
Reacción en Cadena de la Polimerasa
/
Flaviviridae
/
Hepatitis Viral Humana
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos