The rpoN (sigma54) gene from Listeria monocytogenes is involved in resistance to mesentericin Y105, an antibacterial peptide from Leuconostoc mesenteroides.
J Bacteriol
; 179(23): 7591-4, 1997 Dec.
Article
en En
| MEDLINE
| ID: mdl-9393729
To gain insight into the mode of action of mesentericin Y105, a bacteriocin bactericidal agent against Listeria monocytogenes, we undertook to identify the listerial factors mediating this susceptibility by using a genetic approach. Transposon mutants resistant to the bacteriocin were obtained. One of them corresponded to a transposon insertion in a gene (rpoN) encoding a putative protein (447 amino acids) with strong homologies to alternative transcriptional sigma54 factors, including that of Bacillus subtilis (38% identity). Complementation experiments with the wild-type rpoN gene demonstrated that the insertion in rpoN was responsible for the resistance phenotype in L. monocytogenes. Moreover, expression of the L. monocytogenes rpoN gene in an rpoN mutant strain of B. subtilis promoted transcription of a sigma54-dependent operon in the presence of the associated regulator. These results demonstrate that the L. monocytogenes rpoN gene encodes a new sigma54 factor.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Factor sigma
/
Bacteriocinas
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ARN Polimerasas Dirigidas por ADN
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Proteínas de Unión al ADN
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Listeria monocytogenes
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Antibacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos