Differential induction of apoptosis in Swiss 3T3 cells by nitric oxide and the nitrosonium cation.
J Cell Sci
; 110 ( Pt 18): 2315-22, 1997 Sep.
Article
en En
| MEDLINE
| ID: mdl-9378780
We have investigated the effect of nitric oxide (NO) on apoptosis in Swiss 3T3 fibroblasts and compared it to the effect of the nitrosonium cation (NO+). Both species induced apoptosis, confirmed by electron microscopy, propidium iodide staining, DNA laddering and activation of caspases. The kinetics of triggering apoptosis were different for the two redox species: NO+ required only a 2 hour exposure, whereas NO required 24 hours. Three sources of NO were used: aqueous solutions of NO and two NO donors, S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine. The time course of apoptosis induced by these two S-nitrosothiols correlated with their rate of decomposition to NO. The apoptotic effect of NO was reduced in the presence of the NO scavenger oxyhaemoglobin, or the antioxidants N-acetylcysteine and ascorbic acid, whereas in the case of NO+ these antioxidants potentiated apoptosis. Glutathione also had a potentiating effect on the cytotoxicity of NO+. This suggests that cellular antioxidants may play a role in protecting the cell from NO-induced apoptosis while NO+ may trigger apoptosis independently of oxidative stress mechanisms.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cationes
/
Células 3T3
/
Apoptosis
/
Proteínas de Transporte Vesicular
/
Óxido Nítrico
Límite:
Animals
Idioma:
En
Revista:
J Cell Sci
Año:
1997
Tipo del documento:
Article
Pais de publicación:
Reino Unido