The site-specific integration system of the temperate Streptococcus thermophilus bacteriophage phiSfi21.
Virology
; 237(1): 148-58, 1997 Oct 13.
Article
en En
| MEDLINE
| ID: mdl-9344917
The temperate bacteriophage phiSfi21 integrates its DNA into the chromosome of Streptococcus thermophilus strains via site-specific recombination. Nucleotide sequencing of the attachment sites identified a 40-bp identity region which surprisingly overlaps both the 18-terminal bp of the phage integrase gene and the 11-terminal bp of a host tRNAArg gene. A 2.4-kb phage DNA segment, covering attP, the phage integrase, and a likely immunity gene contained all the genetic information for faithful integration of a nonreplicative plasmid into the attB site. A deletion within the int gene led to the loss of integration proficiency. A number of spontaneous deletions were observed in plasmids containing the 2.4-kb phage DNA segment. The deletion sites were localized to the tRNA side of the identity region and to phage or vector DNA with 3- to 6-bp-long repeats from the border region. A similar type of deletion was previously observed in a spontaneous phage mutant.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recombinación Genética
/
Streptococcus
/
Bacteriófagos
/
Integración Viral
Idioma:
En
Revista:
Virology
Año:
1997
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Estados Unidos