Structure of the collagen-binding domain from a Staphylococcus aureus adhesin.

Symersky, J; Patti, J M; Carson, M; House-Pompeo, K; Teale, M; Moore, D; Jin, L; Schneider, A; DeLucas, L J; Höök, M; Narayana, S V

Symersky, J; Patti, J M; Carson, M; House-Pompeo, K; Teale, M; Moore, D; Jin, L; Schneider, A; DeLucas, L J; Höök, M; Narayana, S V.

Afiliación

Symersky J; Center for Macromolecular Crystallography, University of Alabama at Birmingham 35294, USA.

Nat Struct Biol ; 4(10): 833-8, 1997 Oct.

Article en En | MEDLINE | ID: mdl-9334749

RESUMEN

The crystal structure of the recombinant 19,000 M(r) binding domain from the Staphylococcus aureus collagen adhesin has been determined at 2 A resolution. The domain fold is a jelly-roll, composed of two antiparallel beta-sheets and two short alpha-helices. Triple-helical collagen model probes were used in a systematic docking search to identify the collagen-binding site. A groove on beta-sheet I exhibited the best surface complementarity to the collagen probes. This site partially overlaps with the peptide sequence previously shown to be critical for collagen binding. Recombinant proteins containing single amino acid mutations designed to disrupt the surface of the putative binding site exhibited significantly lower affinities for collagen. Here we present a structural perspective for the mode of collagen binding by a bacterial surface protein.

Asunto(s)

Adhesinas Bacterianas/química; Adhesinas Bacterianas/metabolismo; Colágeno/metabolismo; Pliegue de Proteína; Estructura Secundaria de Proteína; Sitios de Unión; Clonación Molecular; Cristalografía por Rayos X; Escherichia coli; Modelos Moleculares; Modelos Estructurales; Señales de Clasificación de Proteína/química; Proteínas Recombinantes/química; Proteínas Recombinantes/metabolismo; Staphylococcus aureus/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colágeno / Estructura Secundaria de Proteína / Pliegue de Proteína / Adhesinas Bacterianas Idioma: En Revista: Nat Struct Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 1997 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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