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Frequent allelic losses of 9p21 markers and low incidence of mutations at p16(CDKN2) gene in non-Hodgkin lymphomas of B-cell lineage.
Fernández-Piqueras, J; Santos, J; Pérez de Castro, I; Meléndez, B; Martínez, B; Robledo, M; Rivas, C; Benítez, J.
Afiliación
  • Fernández-Piqueras J; Departamento de Biología, Facultad de Clencias, Universidad Autónoma de Madrid, Spain.
Cancer Genet Cytogenet ; 98(1): 63-8, 1997 Oct 01.
Article en En | MEDLINE | ID: mdl-9309120
We present an allelotype analysis of 35 cases of non-Hodgkin lymphomas and normal pairs using four microsatellite markers that flank the region occupied by the CDKN2 gene locus at 9p21. Frequent allelic losses (LOH) were detected in B-cell lineage NHLs, including Burkitt lymphoma (33.3% of total, if we only consider high grade tumors). In five of these tumors LOH did not include the CDKN2 gene. Mutational analysis of exon 1 and 2 of CDKN2 (SSGP and sequencing of abnormal bands) revealed a nonsense mutation (Arg72Ter) in one tumor (case 10), where the second hit of the Knudson's model consisted of the elimination of the wild type allele. In view of these results, the hypothesis of two different candidate tumor suppressor gene regions around the CDKN2 locus remains an intriguing possibility.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosomas Humanos Par 9 / Proteínas Portadoras / Linfoma de Células B / Deleción Cromosómica / Alelos / Mutación Tipo de estudio: Incidence_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Cancer Genet Cytogenet Año: 1997 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosomas Humanos Par 9 / Proteínas Portadoras / Linfoma de Células B / Deleción Cromosómica / Alelos / Mutación Tipo de estudio: Incidence_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Cancer Genet Cytogenet Año: 1997 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos