Improved receptor analysis in PET using a priori information from in vitro binding assays.
Phys Med Biol
; 42(8): 1653-60, 1997 Aug.
Article
en En
| MEDLINE
| ID: mdl-9279912
An accurate determination of non-specific binding is required for the analysis of in vitro and in vivo receptor binding data. For some radioligands the non-specific binding is of the same magnitude as the specific binding. Furthermore, in vitro measurements have shown that the non-specific binding can be different in different brain regions. If this is the case in a PET study for determining Bmax and Kd, a correction for the non-specific binding has to be applied. The aim of the present communication is to present a means for determining corrected Bmax and Kd with Scatchard analysis using in vitro binding studies. The influence of non-specific binding on the free and specifically bound radioligand is expressed with the aid of a correction factor, which can be calculated from measurable quantities. Introduction of the corrected free and specifically bound radioligand should give binding parameters closer to reality than previously obtained results.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Unión Radioligante
/
Encéfalo
/
Tomografía Computarizada de Emisión
/
Tomografía Computarizada de Emisión de Fotón Único
/
Receptores de Superficie Celular
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Phys Med Biol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido