IgE response in schistosomiasis japonica: characterization of a cercarial allergen in comparison with purified egg allergens.
J Helminthol
; 71(3): 221-6, 1997 Sep.
Article
en En
| MEDLINE
| ID: mdl-9271469
The relationship between the cercarial allergen and two previously isolated egg allergens (J1, J2) of Schistosoma japonicum was examined especially in terms of the cross-reactivity between them. Semi-purified cercarial allergen (JAC) was obtained from the crude extract of S. japonicum cercariae by gel chromatography on Sephadex G-200. The apparent molecular weight of JAC was estimated approximately as 60-100 kDa. JAC could bind to Con A-Sepharose, indicating its glycoprotein nature. Three groups of BALB/c mice were immunized with JAC , J1 or J2 using A1(OH)3 as adjuvant, and the cross-reactivity of each anti-serum was examined by PCA. Anti-JAC, anti-J1 or anti-J2 serum was highly specific to the corresponding antigen. When IgE-ELISA of S. japonicum patient sera was performed using JAC, J1 or J2 as an antigen, the correlation between anti-J1 and anti-J2 (r = 0.78) was high, whereas the correlation between anti-JAC and anti-J1 (r = 0.27) or between anti-JAC and anti-J2 (r = 0.12) was low. These results suggest that most IgE epitopes on cercarial allergen are independent from those on egg allergens in S. japonicum.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Esquistosomiasis
/
Esquistosomiasis Japónica
/
Inmunoglobulina E
/
Antígenos Helmínticos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Helminthol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido